期刊
OSTEOARTHRITIS AND CARTILAGE
卷 20, 期 11, 页码 1326-1335出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.joca.2012.06.015
关键词
Poly(ethylene glycol); Chondrocyte; Pericellular matrix; Oxidative stress; Photopolymerization
资金
- American Federation for Aging Research
- NIH Pharmaceutical Biotechnology Training Fellowship
- Howard Hughes Medical Institute/Undergraduate Research Opportunities Program fellowship
Objective: The objectives for this study were to determine whether radical initiated photopolymerizations typically employed for cell encapsulations lead to oxidative stress incurred by chondrocytes and whether the development of a pericellular matrix (PCM) decreases this oxidative stress and has longer-term benefits on chondrocyte function. Methods: Freshly isolated bovine chondrocytes were encapsulated in poly(ethylene glycol) (PEG) hythogels devoid of a PCM or with a PCM, confirmed by immunocytochemistry (IC), and cultured for up to 2 weeks. Reactive oxygen species (ROS) production and damage to cell membrane by lipid peroxidation were accomplished using carboxy-2,7-difluorodihydrofluorescein diacetate (carboxy-H(2)DFFDA) and by malondialdehyde (MDA) content, respectively. Gene expression and proteoglycan synthesis were analyzed using reverse transcription (RT)-quantitative PCR (qPCR) and (SO4)-S-35 incorporation, respectively. Results: The photopolymerization reaction, which alone generates radicals and extracellular ROS, led to oxidative stress in chondrocytes evidenced by increased intracellular ROS and lipid peroxidation. The presence of a PCM decreased intracellular ROS and abrogated membrane lipid peroxidation, improved aggrecan, collagen II and collagen VI expression, and enhanced proteoglycan synthesis. Conclusions: The development of the PCM prior to photoencapsulation in PEG hydrogels reduces oxidative stress and improves chondrocyte anabolic activity. Our data suggest this reduction occurs by decreased ROS diffusion into the cell and decreased membrane damage. Our findings suggest that minimizing oxidative stress, such as through the presence of a PCM, may have long-term beneficial effects on tissue elaboration when employing photopolymerizations to encapsulate chondrocytes for cartilage tissue engineering applications. (C) 2012 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.
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