期刊
ORL-JOURNAL FOR OTO-RHINO-LARYNGOLOGY HEAD AND NECK SURGERY
卷 76, 期 3, 页码 127-136出版社
KARGER
DOI: 10.1159/000363575
关键词
Ciliary beat frequency; Topical corticosteroids; Topical antihistamines; Preservatives; Nasal epithelial cells
资金
- National Science Fund for Distinguished Young Scholars [81025007]
- Program for Changjiang Scholars and Innovative Research Team in University [IRT13082]
- National Key Technology R&D Program of the Ministry of Science and Technology [2014BAI07B04]
- National Natural Science Foundation of China [81100704]
- Beijing Natural Science Foundation [7131006]
- Ministry of Health Foundation [201202005]
- Beijing Nova Program [Z111107054511061]
- Specialized Research Fund for the Doctoral Program of Higher Education of China [20111107120004]
- Capital Health Research and Development of Special [2011-1017-03]
- Science Foundation for High-Level Medical Talents of Beijing Health System [2009-02-007]
Background: The aim of this study was to investigate the effect of the corticosteroids, the antihistamines, and the preservatives benzalkonium chloride (BKC) and potassium sorbate (PS) in intranasal medications on human nasal epithelial ciliary beat frequency (CBF). Methods: Primary ciliated epithelial cell cultures from the human nasal mucosa of chronic sinusitis patients were established. Changes in CBF of epithelial cell cultures treated/untreated with intranasal medications or preservatives were assessed using high-speed digital imaging methods. Results: Budesonide caused a rapid but reversible ciliostasis and showed no ciliotoxic effect at 10% dilution. Fluticasone propionate induced an irreversible ciliostatic activity and showed a reversible decrease in CBF at 10% dilution. Azelastine hydrochloride and levocabastine hydrochloride both induced a dose-dependent and irreversible decrease in CBF, although the ciliotoxic effect was not evident at 5% dilution. BKC resulted in an irreversible ciliostasis at 0.005 or 0.01% concentrations, whereas PS did not show any change in CBF at 0.12 or 0.24% concentrations. Conclusions: Crystalline BKC and BKC-containing intranasal medications, including fluticasone propionate, azelastine hydrochloride and levocabastine hydrochloride, but not PS or PS-containing intranasal budesonide spray, led to irreversible ciliostasis in human nasal epithelial cell cultures when applied at clinically relevant concentrations. (C) 2014 S. Karger AG, Basel
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据