期刊
ORGANIC LETTERS
卷 15, 期 19, 页码 5020-5023出版社
AMER CHEMICAL SOC
DOI: 10.1021/ol4023547
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资金
- National Natural Science Foundation of China [21102148, 21125205, 212210017]
- National Basic Research Program of China [2009CB930802, 2011CB935800]
- State Key Laboratory of Fine Chemicals, Department of Chemical Engineering, Dalian University of Technology
A new pH-responsive fluorescent probe has been reported based on protonation-activable resonance charge transfer. In aqueous solution, probe PQ-Lyso exhibits ratiometric detection of pH changes with a large hypsochromic shift of 76 nm and remarkable changes in the fluorescence intensity ratio (R = F-494 (nm)/F-570 (nm), R/R-0 = 105). Furthermore, PQ-Lyso, which is localized to lysosomes in living cells, can calibrate lysosomal pH using fluorescence ratiometry.
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