期刊
ORGANIC LETTERS
卷 15, 期 21, 页码 5528-5530出版社
AMER CHEMICAL SOC
DOI: 10.1021/ol402585c
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资金
- National Natural Science Foundation of China [31100587, 21332006]
- NIH [R01HD065122, R01HD061935]
- NSF [CHE-1012511]
- Natural Science Foundation of Tianjin [12JCYBJC18600]
- Division Of Chemistry
- Direct For Mathematical & Physical Scien [1012511] Funding Source: National Science Foundation
An N-acetylhexosamine 1-kinase from Bifidobacterium infantis (NahK_15697), a guanosine 5'-diphosphate (GDP)-mannose pyrophosphorylase from Pyrococcus furiosus (PFManC), and an Escherichia coli inorganic pyrophosphatase (EcPpA) were used efficiently for a one-pot three-enzyme synthesis of GDP-mannose, GDP-glucose, their derivatives, and GDP-talose. This study represents the first facile and efficient enzymatic synthesis of GDP-sugars and derivatives starting from monosaccharides and derivatives.
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