Pyrrolidinyl Peptide Nucleic Acid Homologues: Effect of Ring Size on Hybridization Properties

The effect of ring size of four- to six-membered cyclic beta-amino acid on the hybridization properties of pyrrolidinyl peptide nucleic acid with an alternating alpha/beta peptide backbone is reported. The cyclobutane derivatives (acbcPNA) show the highest T-m and excellent specificity with cDNA and RNA.