4.8 Article

One- and Two-Photon Live Cell Imaging Using a Mutant SNAP-Tag Protein and Its FRET Substrate Pairs

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ORGANIC LETTERS
卷 13, 期 16, 页码 4160-4163

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AMER CHEMICAL SOC
DOI: 10.1021/ol201430x

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  1. Agency for Science, Technology and Research [R-143-000-391-305]
  2. Ministry of Education [R-143-000-394-112]

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A small molecule-assisted protein labeling strategy based on a mutant SNAP-Tag (mSNAP) and its FRET substrate pairs has been developed. Both one- and two-photon fluorescence microscopic experiments were successfully demonstrated in living cells.

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