期刊
THORAX
卷 70, 期 8, 页码 769-781出版社
BMJ PUBLISHING GROUP
DOI: 10.1136/thoraxjnl-2015-206782
关键词
-
资金
- Italian Ministry of Health
Background Mechanism(s) connecting obstructive sleep apnoea syndrome (OSAS) to liver injury in paediatric non-alcoholic fatty liver disease (NAFLD) are unknown. We hypothesised alterations in gut-liver axis and in the pool and phenotype of hepatic progenitor cells (HPCs) may be involved in OSAS-associated liver injury in NAFLD. Methods Eighty biopsy-proven NAFLD children (age, mean +/- SD, 11.4 +/- 2.0 years, 56% males, body mass index z-score 1.95 +/- 0.57) underwent a clinical-biochemical assessment, with measurement of insulin sensitivity, plasma cytokines, lipopolysaccharide (LPS), an intestinal permeability test and a standard polysomnography. Hepatic toll-like receptor (TLR)-4 expression by liver-resident cells and overall number and expression of resistin and adiponectin by HPCs were assessed by immunofluorescence and immunohistochemistry. OSAS was defined by an apnoea/hypopnoea index >= 1. Results OSAS was characterised by an increased intestinal permeability and endotoxemia, coupled with TLR-4 upregulation in hepatocytes, Kupffer and hepatic stellate cells (HSCs) and by an expansion of an adiponectin-deficient HPC pool, key features of steatohepatitis and fibrosis. The duration of haemoglobin desaturation (SaO(2) <90%) independently predicted intestinal permeability (beta: 0.396; p=0.026), plasma LPS (beta: 0.358; p=0.008) and TLR-4 expression by hepatocytes (beta: 0.332; p=0.009), Kupffer cells (beta: 0.357; p=0.006) and HSCs (beta:0.445; p=0.002). SaO(2) <90% predicted also HPC number (beta: 0.471; p=0.001) and impaired adiponectin expression by HPC pool (beta: -0.532; p=0.0009). These relationships were observed in obese and nonobese children. Conclusions In paediatric NAFLD, OSAS is associated with increased endotoxemia coupled with impaired gut barrier function, with increased TLR-4-mediated hepatic susceptibility to endotoxemia and with an expansion of an adiponectin-deficient HPC pool. These alterations may represent a novel pathogenic link and a potential therapeutic target for OSAS-associated liver injury in NAFLD.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据