The base discriminating potential of pyrrolidinyl PNA demonstrated by magnetic FexOy particles

Pyrrolidinyl PNA was immobilized on FexOy magnetic particles and was able to capture and thereby discriminate single base alterations in DNA counterstrands better than DNA. The selectivities of matched vs. mismatched oligonucleotides measured by the absorption differences were up to 10-12 which are remarkable values for linear probes.