期刊
ORGANIC & BIOMOLECULAR CHEMISTRY
卷 10, 期 17, 页码 3379-3383出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c2ob25258a
关键词
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资金
- NIHR Biomedical Research Centre, Oxford, UK
- Swedish Research Council
- Loo and Hans Ostermanns Foundation for Geriatric Research
- Foundation for Geriatric Diseases at the Karolinska Institutet
- Medical Research Council [G0501068] Funding Source: researchfish
- MRC [G0501068] Funding Source: UKRI
Novel ubiquitin-based active site probes including a fluorescent tag have been developed and evaluated. A new, functionalizable electrophilic trap is utilized allowing for late stage diversification of the probe. Attachment of fluorescent dyes allowed direct detection of endogenous deubiquitinating enzyme (DUB) activities in cell extracts by in-gel fluorescence imaging.
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