期刊
ORGANIC & BIOMOLECULAR CHEMISTRY
卷 9, 期 19, 页码 6727-6733出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c1ob05565h
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资金
- German Federal Ministry of Education and Research (BMBF)
- BASF SE (Ludwigshafen, Germany)
The oxofunctionalization of saturated hydrocarbons is an important goal in basic and applied chemistry. Biocatalysts like cytochrome P450 enzymes can introduce oxygen into a wide variety of molecules in a very selective manner, which can be used for the synthesis of fine and bulk chemicals. Cytochrome P450 enzymes from the CYP153A subfamily have been described as alkane hydroxylases with high terminal regioselectivity. Here we report the product yields resulting from C-5-C-12 alkane and alcohol oxidation catalyzed by CYP153A enzymes from Mycobacterium marinum (CYP153A16) and Polaromonas sp. (CYP153A P. sp.). For all reactions, byproduct formation is described in detail. Following cloning and expression in Escherichia coli, the activity of the purified monooxygenases was reconstituted with putidaredoxin (CamA) and putidaredoxin reductase (CamB). Although both enzyme systems yielded primary alcohols and alpha,omega-alkanediols, each one displayed a different oxidation pattern towards alkanes. For CYP153A P. sp. a predominant omega-hydroxylation activity was observed, while CYP153A16 possessed the ability to catalyze both omega-hydroxylation and alpha,beta-dihydroxylation reactions.
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