Sequential two-step multienzyme synthesis of tumor-associated sialyl T-antigens and derivatives

A series of alpha 2-3-sialylated beta 1-3-linked galactosides, including sialyl T-antigens, 3'-sialyl galacto-N-biose, 3'-sialyl lacto-N-biose, and their derivatives containing natural and non-natural sialic acid forms have been synthesized from simple monosaccharides using an efficient sequential two-step multienzyme approach.