期刊
ORGANIC & BIOMOLECULAR CHEMISTRY
卷 9, 期 24, 页码 8356-8370出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c1ob05637a
关键词
-
资金
- Swedish Foundation for Strategic Research
- Knut and Alice Wallenberg foundation
- European Union
- ERC
- European Research Council
- Knight Alzheimer's Disease Research Center (NIH) [P50AG05681, 5PO1-AG03991]
- NATIONAL INSTITUTE ON AGING [P01AG003991, P50AG005681] Funding Source: NIH RePORTER
Molecular probes for selective identification of protein aggregates are important to advance our understanding of the molecular pathogenesis underlying protein aggregation diseases. Here we report the chemical design of a library of anionic luminescent conjugated oligothiophenes (LCOs), which can be utilized as ligands for detection of protein aggregates. Certain molecular requirements were shown to be necessary for detecting (i) early non-thioflavinophilic protein assemblies of A beta 1-42 and insulin preceding the formation of amyloid fibrils and (ii) for obtaining distinct spectral signatures of the two main pathological hallmarks observed in human Alzheimer's diease brain tissue (A beta plaques and neurofibrillary tangles). Our findings suggest that a superior anionic LCO-based ligand should have a backbone consisting of five to seven thiophene units and carboxyl groups extending the conjugated thiophene backbone. Such LCOs will be highly useful for studying the underlying molecular events of protein aggregation diseases and could also be utilized for the development of novel diagnostic tools for these diseases.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据