期刊
ORGANIC & BIOMOLECULAR CHEMISTRY
卷 7, 期 7, 页码 1404-1409出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/b819946a
关键词
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资金
- Danish National Research Foundation
- Danish National Advanced Technology Foundation
Three different LNA-nucleoside triphosphates, LNA-TTP, LNA-ATP and LNA-5-methyl-CTP, were investigated as substrates for KOD DNA polymerase. The results reveal that KOD DNA polymerase is an efficient catalyst for template directed synthesis of DNA oligonucleotide duplexes containing a large number of LNA nucleotides by primer extension reactions. Furthermore, KOD DNA polymerase is shown to be suitable for the PCR amplification of an LNA-modified DNA duplex.
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