期刊
ORGANIC & BIOMOLECULAR CHEMISTRY
卷 6, 期 22, 页码 4125-4133出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/b811427g
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资金
- Swiss National Science Foundation [200020_119866/1]
- Swiss Office for Science and Education [SER C07.0116]
The cellular uptake mechanism and intracellular distribution of emissive lanthanide helicates have been elucidated by time-resolved luminescence microscopy (TRLM). The helicates are non-cytotoxic and taken up by normal (HaCat) and cancer (HeLa, MCF-7) cells by endocytosis and show a late endosomal-lysosomal cellular distribution. The lysosomes predominantly localize around the nucleus and co-localize with the endoplasmatic reticulum. The egress is slow and limited, around 30% after 24 h. The first bright luminescent images can be observed with an external concentration gradient of 5 mu M of the Eu-III helicate [Q = 0.21, tau = 2.43 ms], compared to > 10 mM when using conventional luminescence microscopy. Furthermore, multiplex labeling could be achieved with the Tb-III [Q = 0.11, tau = 0.65 ms], and Sm-III [Q = 0.0038, tau = 0.030 ms] analogues.
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