期刊
ORAL ONCOLOGY
卷 49, 期 2, 页码 129-135出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.oraloncology.2012.08.003
关键词
Epidermal growth factor receptor; HMG box-containing protein 1; N-acetylcysteine; Oral cancer
资金
- China Medical University [CMU100-TC-21]
- National Science Council [NSC96-2321-B-039-006-MY2, NSC98-2320-B-309-002-MY3]
Objectives: Overexpression of the epidermal growth factor (EGF) receptor (EGFR) gene in the squamous cell carcinomas of the head and neck (SCCHN) is often associated with inauspicious prognosis and poor survival. N-acetylcysteine (NAC), a compound from some vegetables and allium species, appears anti-tumorigenesis, but the underlying mechanism is unclear. The objective of this study is to investigate the role of NAC in EGFR-overexpressing oral cancer. Materials and methods: Both HSC-3 and SCC-4 human tongue squamous carcinoma cell lines and an HSC-3 xenograft mouse model were used to test the anti-growth efficacy of NAC in vitro and in vivo, respectively. Results: NAC treatment suppressed cell growth, with concomitantly increased expression of HMG box-containing protein 1 (HBP1), a transcription suppressor, and decreased EGFR/Akt activation, in EGFR-overexpressing HSC-3 oral cancer cells. HBP1 knockdown attenuated the growth arrest and apoptosis induced by NAC. Lastly, NAC and AG1478, an EGFR inhibitor, additively suppressed colony formation in HSC-3 cells. Conclusion: Taken together, our data indicate that NAC exerts its growth-inhibitory function through modulating EGFR/Akt signaling and HBP1 expression in EGFR-overexpressing oral cancer. (C) 2012 Elsevier Ltd. All rights reserved.
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