期刊
ORAL DISEASES
卷 16, 期 1, 页码 46-54出版社
WILEY
DOI: 10.1111/j.1601-0825.2009.01602.x
关键词
bone marrow stromal cells; bone morphogenetic proteins; gene therapy; tissue engineering; mandibular defects
资金
- National Natural Science Foundation of China [30400502, 30772431]
- Program for New Century Excellent Talents in University [NCET-08-0353]
- Science and Technology Commission of Shanghai Municipality [07DZ22007, 08410706400, 08JC1414400, 08DZ2271100, 08QH14017, S30206]
- Shanghai Rising-star Program [08QH14017, 05QMX1426]
- Shanghai Education Committee [07SG19]
Objective: To investigate the effects of mandibular defects repaired by a tissue engineered bone complex with beta-tricalcium phosphate (beta-TCP) and bone morphogenic protein-2 (BMP-2) gene-modified bone marrow stromal cells (bMSCs). Materials and methods: bMSCs derived from Fisher 344 rats were cultured and transduced with adenovirus AdBMP-2, AdEGFP gene in vitro. Osteogenic differentiation of bMSCs was determined by alkaline phosphatase staining, von Kossa assay and reverse transcription-polymerase chain reaction. Gene transduced or untransduced bMSCs were seeded on beta-TCP scaffolds to repair mandibular full thickness defects with a diameter of 5 mm. Eight weeks post-operation, X-ray examination, micro-computerized tomography and histological and histomorphological analysis were used to evaluate the bone healing effects. Results: Alkaline phosphatase staining and mineralized nodules formation were more pronounced in AdBMP-2 group 14 days after gene transduction when compared with that of AdEGFP or untransduced group. The mRNA expression of osteopontin and osteocalcin also significantly increased 9 days after AdBMP-2 gene transduction. Mandibular defects were successfully repaired with AdBMP-2-transduced bMSCs/beta-TCP constructs. The percentage of new bone formation in AdBMP-2 group was significantly higher than that of other control groups. Conclusions: Bone morphogenic protein-2 regional gene therapy together with beta-TCP scaffold could be used to promote mandibular repairing and bone regeneration.
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