期刊
OPTICS LETTERS
卷 39, 期 18, 页码 5431-5434出版社
OPTICAL SOC AMER
DOI: 10.1364/OL.39.005431
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资金
- NIH/NIBIB [R00 EB008557]
- NIH/NIDCR [R01 DE023497]
- NIH/NCI [R01 CA175391]
- Division Of Human Resource Development
- Direct For Education and Human Resources [1311318] Funding Source: National Science Foundation
We have previously developed a line-scanned dual-axis confocal (LS-DAC) microscope with subcellular resolution suitable for high-frame-rate diagnostic imaging at shallow depths. Due to the loss of confocality along one dimension, the contrast (signal-to-background ratio) of a LS-DAC microscope is deteriorated compared to a point-scanned DAC microscope. However, by using a sCMOS camera for detection, a short oblique light-sheet is imaged at each scanned position. Therefore, by scanning the light sheet in only one dimension, a thin 3D volume is imaged. Both sequential two-dimensional deconvolution and three-dimensional deconvolution are performed on the thin image volume to improve the resolution and contrast of one en face confocal image section at the center of the volume, a technique we call sheet-scanned dual-axis confocal (SS-DAC) microscopy. (C) 2014 Optical Society of America
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