期刊
OPTICS LETTERS
卷 39, 期 1, 页码 108-110出版社
OPTICAL SOC AMER
DOI: 10.1364/OL.39.000108
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- Army Research Office [W911NF-12-2-0024, W911NF-12-1-0325]
We report on ultralow frequency Stokes and anti-Stokes Raman spectroscopy of single living cells and microsized particles in an aqueous medium with a frequency shift down to 10 cm(-1) by the combination of a hot rubidium (Rb) vapor filter, a confocal pinhole, and optical trapping. A single frequency-stabilized diode laser beam at 780.2 nm is used to optically trap and excite a single living cell or microparticle, and the Rayleigh scattering light from the particle is effectively blocked with a Rb vapor cell and a confocal pinhole. Ultralow frequency Raman spectra of the trapped cells or microparticles in both Stokes and anti-Stokes regions are then measured with a single-stage CCD spectrograph. (C) 2013 Optical Society of America
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