期刊
OPTICS LETTERS
卷 36, 期 7, 页码 1137-1139出版社
OPTICAL SOC AMER
DOI: 10.1364/OL.36.001137
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资金
- National Institutes of Health (NIH) [R01 EB000712, R01 EB008085, R01 CA134539, U54 CA136398, R01 CA157277, P41-EB2182]
- Microphotoacoustics, Inc.
- Endra, Inc.
Photoacoustic microscopy (PAM) offers label-free, optical absorption contrast. A high-speed, high-resolution PAM system in an inverted microscope configuration with a laser pulse repetition rate of 100, 000 Hz and a stationary ultrasonic transducer was built. Four-dimensional in vivo imaging of microcirculation in mouse skin was achieved at 18 three-dimensional volumes per second with repeated two-dimensional (2D) raster scans of 100 by 50 points. The corresponding 2D B-scan (50 A-lines) frame rate was 1800 Hz, and the one-dimensional A-scan rate was 90, 000 Hz. The lateral resolution is 0.23 +/- 0.03 mu m for Au nanowire imaging, which is 2.0 times below the diffraction limit. (C) 2011 Optical Society of America
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