期刊
OPTICS LETTERS
卷 35, 期 10, 页码 1521-1523出版社
OPTICAL SOC AMER
DOI: 10.1364/OL.35.001521
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资金
- National Institutes of Health (NIH) [R01 EB000712, EB000712A2S1, R01 EB00071207S2, R01 EB008085, R01 CA113453901, U54 CA136398, 5P60 DK02057933]
Recently developed optical-resolution photoacoustic microscopy (OR-PAM), which is based on the detection of optical absorption contrast, is complementary to other optical microscopy modalities such as optical confocal microscopy, optical coherence tomography, and multiphoton microscopy. A hybrid optical-mechanical scanning configuration increases the imaging speed of OR-PAM significantly, facilitating many demanding biomedical applications. With a high-pulse-repetition-rate laser, the hybrid-scanning OR-PAM can acquire one-dimensional depth-resolved images (A-lines) at 5 kHz and two-dimensional B-scan images containing 800 A-lines at 6.25 Hz. We demonstrated in vivo in a mouse three-dimensional imaging of the iris vasculature in 128 s for an 800 x 800 x 200 data set and of the ear vasculature in 256 s for an 800 x 1600 x 200 data set. (C) 2010 Optical Society of America
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