期刊
OPTICS LETTERS
卷 34, 期 16, 页码 2495-2497出版社
OPTICAL SOC AMER
DOI: 10.1364/OL.34.002495
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资金
- Engineering and Physical Sciences Research Council (EPSRC) Advanced Research Fellow [EP/E055818/1]
- Biotechnology and Biological Sciences Research Council (BBSRC) [BBE0049461]
- BBSRC [BB/E004946/1, BB/F011512/1] Funding Source: UKRI
- EPSRC [EP/E055818/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/F011512/1, BB/E004946/1] Funding Source: researchfish
- Engineering and Physical Sciences Research Council [EP/E055818/1] Funding Source: researchfish
We demonstrate wavefront sensorless aberration correction in a two-photon excited fluorescence microscope. Using analysis of the image-formation process, we have developed an optimized correction scheme permitting image-quality improvement with minimal additional exposure of the sample. We show that, as a result, our correction process induces little photobleaching and significantly improves the quality of images of biological samples. In particular, increased visibility of small structures is demonstrated. Finally, we illustrate the use of this technique on various fresh and fixed biological tissues. (C) 2009 Optical Society of America
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