期刊
OPTICS LETTERS
卷 34, 期 20, 页码 3154-3156出版社
OPTICAL SOC AMER
DOI: 10.1364/OL.34.003154
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资金
- Biological Science Research Council [BBE0049461]
- Engineering and Physical Sciences Research Council [EP/E055818/1]
- Austrian Science Fund [J2826-N20]
- Biotechnology and Biological Sciences Research Council [BB/E004946/1, BB/F011512/1] Funding Source: researchfish
- Engineering and Physical Sciences Research Council [EP/E055818/1] Funding Source: researchfish
- BBSRC [BB/E004946/1, BB/F011512/1] Funding Source: UKRI
- EPSRC [EP/E055818/1] Funding Source: UKRI
Adaptive optics is implemented in a harmonic generation microscope using a wavefront sensorless correction scheme. Both the second- and third-harmonic intensity signals are used as the optimization metric. Aberration correction is performed to compensate both system- and specimen-induced aberrations by using an efficient optimization routine based upon Zernike polynomial modes. Images of live mouse embryos show an improved signal level and resolution. (C) 2009 Optical Society of America
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