期刊
OPTICS EXPRESS
卷 22, 期 17, 页码 20663-20677出版社
OPTICAL SOC AMER
DOI: 10.1364/OE.22.020663
关键词
-
类别
We describe a two-beam interference structured illumination fluorescence microscope. The novelty of the presented system lies in its simplicity. A programmable spatial light modulator (ferroelectric LCoS) in an intermediate image plane enables precise and rapid control of the excitation pattern in the specimen. The contrast of the projected light pattern is strongly influenced by the polarization state of the light entering the high NA objective. To achieve high contrast, we use a segmented polarizer. Furthermore, a mask with six holes blocks unwanted components in the spatial frequency spectrum of the illumination grating. Both these passive components serve their purpose in a simpler and almost as efficient way as active components. We demonstrate a lateral resolution of 114.2 +/- 9.5 nm at a frame rate of 7.6 fps per reconstructed 2D slice. (C)2014 Optical Society of America
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据