High throughput multichannel fluorescence microscopy with microlens arrays

We present a multichannel fluorescence microscopy technique for high throughput imaging applications. A microlens array with over 140,000 elements is used to image centimeter-scale samples at up to 18.1 megapixels per second. Large field-of-view multichannel fluorescent imaging is demonstrated in both sequential and parallel geometries. The extended dynamic range of this approach is also discussed. (C) 2014 Optical Society of America