4.6 Article

An in vitro investigation of the actions of reproductive hormones on the cervix of the ewe in the follicular stage: The effects of 17β-estradiol, oxytocin, FSH, and arachidonic acid on the cervical pathway for the synthesis of prostaglandin E2

期刊

THERIOGENOLOGY
卷 83, 期 6, 页码 1007-1014

出版社

ELSEVIER SCIENCE INC
DOI: 10.1016/j.theriogenology.2014.12.003

关键词

Cervix; Oxytocin receptor; Cytoplasmic phospholipase A(2); Prostaglandin E-2; Sheep; In vitro

资金

  1. Regione Autonoma della Sardegna, Programma Master and Back, Italy
  2. European Union [MEXC-CT-2006-042499]
  3. European Union Marie Curie Chair of Excellence [MEXCCT-2006-042499]

向作者/读者索取更多资源

During the periovulatory period, the cervix of the ewe relaxes and this mechanism is thought to be mediated by oxytocin and prostaglandin E-2 (PGE(2)) in response to increased concentrations of 17 beta-estradiol and perhaps FSH. The aim of the study was to determine the in vitro effects of 17 beta-estradiol, FSH, oxytocin, and arachidonic acid (AA) on the synthesis of PGE(2) and on the expression of oxytocin receptor (OTR), cytoplasmic phospholipase A(2) (cPLA(2)), and cyclooxygenase 2 (COX-2) in explants of cervical tissue collected from ewes in the periovulatory phase of the estrous cycle. Cervical minces from ewes in the follicular phase of the estrous cycle were cultured in supplemented Eagle's Minimum Essential Medium for 48 hours with 17 beta-estradiol, FSH, oxytocin, or AA. After incubation, the tissue was stored at -80 degrees C and the media at -20 degrees C. Western immunoblotting was used to determine relative levels of OTR, cPLA(2), and COX-2 in cervical tissue, and the media was analyzed by RIA, to determine the concentration of PGE(2). The addition of 17 beta-estradiol increased the concentration of PGE(2) in the media (P = 0.001), the levels of COX-2 (P = 0.02) and OTR (P = 0.006) but not those of cPLA(2) (P = 0.15). The addition of FSH increased the levels of COX-2 (P = 0.01) but, it had no effect on the concentration of PGE(2) (P = 0.08) or on the levels of OTR (P = 0.07) and cPLA(2) (P = 0.15). Oxytocin did not increase the levels of COX-2 (P = 0.38) but increased those of OTR (P = 0.001) and cPLA(2) (P = 0.01) but not on the concentration of PGE(2) in the media. Arachidonic acid increased the levels of cPLA(2) (P = 0.01) and those of COX-2 (P = 0.02) but not the concentration of PGE(2) in the media. Our findings suggest that the PGE(2)-mediated mechanisms of cervical relaxation in the ewe during the follicular phase are stimulated by FSH, 17 beta-estradiol, oxytocin, and AA. They all appear to act by inducing receptors and enzymes along the synthetic pathway for PGE(2). (C) 2015 Elsevier Inc. All rights reserved.

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