期刊
OPTICS EXPRESS
卷 20, 期 16, 页码 17741-17759出版社
OPTICAL SOC AMER
DOI: 10.1364/OE.20.017741
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资金
- National Basic Research Program of China [2011CB910401]
- Science Fund for Creative Research Group of China [61121004]
- National Natural Science Foundation of China [30970691]
- Program for New Century Excellent Talents in University of China [NCET-10-0407]
- Fundamental Research Funds for the Central Universities [2011TS087]
Nowadays, there is a hot debate among industry and academic researchers that whether the newly developed scientific-grade Complementary Metal Oxide Semiconductor (sCMOS) cameras could become the image sensors of choice in localization-based super-resolution microscopy. To help researchers find answers to this question, here we reported an experimental methodology for quantitatively comparing the performance of low-light cameras in single molecule detection (characterized via image SNR) and localization (via localization accuracy). We found that a newly launched sCMOS camera can present superior imaging performance than a popular Electron Multiplying Charge Coupled Device (EMCCD) camera in a signal range (15-12000 photon/pixel) more than enough for typical localization-based super-resolution microscopy. (C) 2012 Optical Society of America
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