期刊
OPTICS EXPRESS
卷 20, 期 19, 页码 20998-21009出版社
OPTICAL SOC AMER
DOI: 10.1364/OE.20.020998
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资金
- Wellcome Trust [095927/A/11/Z, 095927/B/11/Z]
- Kavli Foundation
- National Institute of General Medical Sciences [F32GM096859]
- Engineering and Physical Sciences Research Council, UK [EP/E055818/1]
- EPSRC [EP/E055818/1] Funding Source: UKRI
- Wellcome Trust [095927/B/11/Z] Funding Source: Wellcome Trust
- Engineering and Physical Sciences Research Council [EP/E055818/1] Funding Source: researchfish
Stimulated emission depletion (STED) microscopy allows fluorescence far-field imaging with diffraction-unlimited resolution. Unfortunately, extending this technique to three-dimensional (3D) imaging of thick specimens has been inhibited by sample-induced aberrations. Here we present the first implementation of adaptive optics in STED microscopy to allow 3D super-resolution imaging in strongly aberrated imaging conditions, such as those introduced by thick biological tissue. (C) 2012 Optical Society of America
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