期刊
OPTICS EXPRESS
卷 19, 期 4, 页码 3396-3405出版社
OPTICAL SOC AMER
DOI: 10.1364/OE.19.003396
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资金
- Korea Science and Engineering Foundation (KOSEF)/Ministry of Education, Science and Technology
- National Research Foundation of Korea [2006-0050690] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
We demonstrate a high-speed confocal fluorescence lifetime imaging microscopy (FLIM) whose accuracy and photon economy are as good as that of a time-correlated single photon counting (TCSPC). It is based on a new lifetime determination scheme, the analog mean delay (AMD) method. Due to the technical advantages of multiple fluorescence photon detection capability, accurate lifetime determination scheme and high photon detection efficiency, the AMD method can be the most effective method for high-speed confocal FLIM. The feasibility of real-time confocal FLIM with the AMD method has been demonstrated by observing the dynamic reaction of calcium channels in a RBL-2H3 cell with respect to 4 alpha PDD stimulus. We have achieved the photon detection rate of 125 times faster than a conventional TCSPC based system in this experiment. (C) 2011 Optical Society of America
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