期刊
OPTICS EXPRESS
卷 18, 期 13, 页码 13720-13744出版社
OPTICAL SOC AMER
DOI: 10.1364/OE.18.013720
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资金
- MRC [G0400598]
- EPSRC [EP/D501199/1]
- UCL
- UCLB
- Wellcome Trust [064413]
- Worshipful Company of Scientific Instrument Makers
- EPSRC [EP/D501199/1] Funding Source: UKRI
- MRC [G0400598] Funding Source: UKRI
- Engineering and Physical Sciences Research Council [EP/D501199/1] Funding Source: researchfish
- Medical Research Council [G0400598] Funding Source: researchfish
We describe a high speed 3D Acousto-Optic Lens Microscope (AOLM) for femtosecond 2-photon imaging. By optimizing the design of the 4 AO Deflectors (AODs) and by deriving new control algorithms, we have developed a compact spherical AOL with a low temporal dispersion that enables 2-photon imaging at 10-fold lower power than previously reported. We show that the AOLM can perform high speed 2D raster-scan imaging (>150 Hz) without scan rate dependent astigmatism. It can deflect and focus a laser beam in a 3D random access sequence at 30 kHz and has an extended focusing range (>137 mu m; 40X 0.8NA objective). These features are likely to make the AOLM a useful tool for studying fast physiological processes distributed in 3D space (C) 2010 Optical Society of America
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