期刊
OPTICS EXPRESS
卷 18, 期 10, 页码 10510-10523出版社
OPTICAL SOC AMER
DOI: 10.1364/OE.18.010510
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资金
- Okawa Foundation
- Vodafone Americas Foundation
- DARPA DSO [56556-MS-DRP]
- NSF BISH [0754880, 0930501]
- Office of The Director, National Institutes of Health [DP2OD006427]
- AFOSR [08NE255]
- Office of Naval Research (ONR)
- Directorate For Engineering
- Div Of Chem, Bioeng, Env, & Transp Sys [0930501] Funding Source: National Science Foundation
- Div Of Chem, Bioeng, Env, & Transp Sys
- Directorate For Engineering [0954482, 0754880] Funding Source: National Science Foundation
We demonstrate the use of a compressive sampling algorithm for on-chip fluorescent imaging of sparse objects over an ultra-large field-of-view (>8 cm(2)) without the need for any lenses or mechanical scanning. In this lensfree imaging technique, fluorescent samples placed on a chip are excited through a prism interface, where the pump light is filtered out by total internal reflection after exciting the entire sample volume. The emitted fluorescent light from the specimen is collected through an on-chip fiber-optic faceplate and is delivered to a wide field-of-view opto-electronic sensor array for lensless recording of fluorescent spots corresponding to the samples. A compressive sampling based optimization algorithm is then used to rapidly reconstruct the sparse distribution of fluorescent sources to achieve similar to 10 mu m spatial resolution over the entire active region of the sensor-array, i.e., over an imaging field-of-view of >8 cm(2). Such a wide-field lensless fluorescent imaging platform could especially be significant for high-throughput imaging cytometry, rare cell analysis, as well as for micro-array research. (C) 2010 Optical Society of America
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