期刊
OPTICS EXPRESS
卷 17, 期 15, 页码 13222-13235出版社
OPTICAL SOC AMER
DOI: 10.1364/OE.17.013222
关键词
-
类别
资金
- National Science Foundation [CBET 0754122]
- National Institutes of Health [PHS 1 R01 CA118562]
- Directorate For Engineering
- Div Of Chem, Bioeng, Env, & Transp Sys [754122] Funding Source: National Science Foundation
We report on the design and demonstration of an optical imaging system capable of exciting surface-bound fluorophores within the resonant evanescent electric field of a photonic crystal surface and gathering fluorescence emission that is directed toward the imaging objective by the photonic crystal. The system also has the ability to quantify shifts in the local resonance angle induced by the adsorption of biomolecules on the photonic crystal surface for label-free biomolecular imaging. With these two capabilities combined within a single detection system, we demonstrate label-free images self-registered to enhanced fluorescence images with 328x more sensitive fluorescence detection relative to a glass surface. This technique is applied to a DNA microarray where label-free quantification of immobilized capture DNA enables improved quality control and subsequent enhanced fluorescence detection of dye-tagged hybridized DNA yields 3x more genes to be detected versus commercially available microarray substrates. (C) 2009 Optical Society of America
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据