期刊
OPTICS EXPRESS
卷 17, 期 8, 页码 6421-6435出版社
OPTICAL SOC AMER
DOI: 10.1364/OE.17.006421
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资金
- Swiss National Science Foundation [3100A0-114624]
- National Center of Competence in Research
Nonlinear microscopy techniques crucially rely on efficient signal detection. Here, we present a ring of large-core optical fibers for epicollection of fluorescence photons that are not transmitted through the objective and thus normally wasted. Theoretical treatments indicated that such a supplementary fiber-optic light collection system (SUFICS) can provide an up to 4-fold signal gain. In typical in vivo imaging experiments, the fiber-ring channel was brighter than the objective channel down to 800 mu m depth, thus providing a gain >2. Moreover, SUFICS reduced noise levels in calcium imaging experiments by about 23%. We recommend SUFICS as a generally applicable, effective add-on to nonlinear microscopes for enhancing fluorescence signals. (C) 2009 Optical Society of America
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