期刊
OPTICS EXPRESS
卷 17, 期 8, 页码 6209-6217出版社
OPTICAL SOC AMER
DOI: 10.1364/OE.17.006209
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资金
- National Institutes of Health [1U54-RR022232, R01 NS28695, R01 NS051786]
- National Science Foundation [DBI-0619674]
We describe open-loop and closed-loop multiplexed force measurements using holographic optical tweezers. We quantify the performance of our novel video-based control system in a driven suspension of colloidal particles. We demonstrate our system's abilities with the measurement of the mechanical coupling between Aplysia bag cell growth cones and beads functionalized with the neuronal cell adhesion molecule, apCAM. We show that cells form linkages which couple beads to the underlying cytoskeleton. These linkages are intermittent, stochastic and heterogeneous across beads distributed near the leading edge of a single growth cone. (C) 2009 Optical Society of America
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