期刊
THEORETICAL AND APPLIED GENETICS
卷 128, 期 6, 页码 1039-1047出版社
SPRINGER
DOI: 10.1007/s00122-015-2488-y
关键词
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资金
- Australian Research Council [LP0882095, LP0883462, LP110100200, DP0985953]
- Australian India Strategic Research Fund (AISRF) Grand Challenge fund [GCF010013]
- CGIAR Generation Challenge Programme (Theme Leader Discretionary grant)
- Australian Genome Research Facility (AGRF)
- Queensland Cyber Infrastructure Foundation (QCIF)
- Australian Partnership for Advanced Computing (APAC)
- Center of Excellence in Genomics (CEG) of ICRISAT
- Australian Research Council [LP110100200] Funding Source: Australian Research Council
We characterise the distribution of crossover and non-crossover recombination in Brassica napus and Cicer arietinum using a low-coverage genotyping by sequencing pipeline SkimGBS. The growth of next-generation DNA sequencing technologies has led to a rapid increase in sequence-based genotyping for applications including diversity assessment, genome structure validation and gene-trait association. We have established a skim-based genotyping by sequencing method for crop plants and applied this approach to genotype-segregating populations of Brassica napus and Cicer arietinum. Comparison of progeny genotypes with those of the parental individuals allowed the identification of crossover and non-crossover (gene conversion) events. Our results identify the positions of recombination events with high resolution, permitting the mapping and frequency assessment of recombination in segregating populations.
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