Detailed mapping of a resistance locus against Fusarium wilt in cultivated eggplant (Solanum melongena)

Key message This is the first report on genetic mapping of a resistance locus against Fusarium wilt caused by the plant pathogen Fusarium oxysporum f. sp. melongenae in cultivated eggplant. Fusarium wilt, caused by the plant pathogen Fusarium oxysporum f. sp. melongenae, is a major soil-borne disease threatening stable production in eggplant (Solanum melongena). Although three eggplant germplasms, LS1934, LS174, and LS2436, are known to be highly resistant to the pathogen, their resistance loci have not been mapped. In this study, we performed quantitative trait locus analyses in F-2:3 populations and detected a resistance locus, FM1, at the end of chromosome 2, with two alleles, Fm1 (L) and Fm1 (E) , in the F-2 populations LWF2 [LS1934 x WCGR112-8 (susceptible)] and EWF2 [EPL-1 (derived from LS174) x WCGR112-8], respectively. The percentage of phenotypic variance explained by Fm1 (L) derived from LS1934 was 75.0 % [Logarithm of the odds (LOD) = 29.3], and that explained by Fm1 (E) derived from EPL-1 was 92.2 % (LOD = 65.8). Using backcrossed inbred lines, we mapped FM1 between two simple sequence repeat markers located similar to 4.881 cM apart from each other. Comparing the location of the above locus to those of previously reported ones, the resistance locus Rfo-sa1 from an eggplant ally (Solanum aethiopicum gr. Gilo) was mapped very close to FM1, whereas another resistance locus, from LS2436, was mapped to the middle of chromosome 4. This is the first report of mapping of a Fusarium resistance locus in cultivated eggplant. The availability of resistance-linked markers will enable the application of marker-assisted selection to overcome problems posed by self-incompatibility and introduction of negative traits because of linkage drag, and will lead to clear understanding of genetic mechanism of Fusarium resistance.