期刊
ONCOLOGY RESEARCH
卷 20, 期 11, 页码 517-528出版社
COGNIZANT COMMUNICATION CORP
DOI: 10.3727/096504013X13747716581336
关键词
Hepatocellular carcinoma (HCC); Mouse model; Diethylnitrosamine (DEN); Carbon tetrachloride (CCl4); Ethanol
类别
资金
- National Natural Science Foundation of China [81060203]
The aim of this study was to develop an efficient and reproducible mouse model for hepatocellular carcinoma (HCC) research and assess the expression of two proto-oncogenes (c-myc and N-ras) and tumor suppressor gene p53 in the carcinogenic process. In this study, we found that diethylnitrosamine initiation with CCl4 and ethanol promotion could induce a short-term, two-stage liver carcinogenesis model in male BALB/c mice, the process of hepatocarcinogenesis including liver damage, liver necrosis/cell death, liver inflammation, liver proliferation, liver hyperplasia, liver steatosis, and liver cirrhosis and hepatocellular nodules, which mimicked the usual sequence of events observed in human HCC. We also identified that the increase in expression of the p53 gene is related to the proliferation of hepatocytes, whereas overexpression of the c-myc and N-ras genes is associated with hepatocarcinogenesis. This animal model may serve as a basis for recapitulating the molecular pathogenesis of HCC seen in humans.
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