期刊
ONCOLOGY REPORTS
卷 29, 期 4, 页码 1415-1420出版社
SPANDIDOS PUBL LTD
DOI: 10.3892/or.2013.2263
关键词
papillary thyroid carcinoma; miRNA; biomarker; gene profile
类别
资金
- NSFC [30770649, 30970682]
- Doctoral Program of Higher Education of China [20100061110070]
- Program for New Century Excellent Talents in the University
- Jilin Provincial Science and Technology Development Project [201205008]
- Science and Technology Project of the Department of Health of Jilin Province [2010Z005]
- Jilin University Doctoral Interdisciplinary Scientific Research Project [450060483092]
The incidence of thyroid cancer has recently experienced a rapid increase in China, and papillary thyroid carcinoma (PTC) accounts for nearly 80% of human thyroid cancers. In the present study, the differential expression of microRNAs (miRNAs) and their target genes were identified in order to analyze the potential roles of miRNAs as biomarkers and in papillary thyroid carcinogenesis. One hundred and twenty-six PTC samples were collected from patients at the China-Japan Union Hospital, China, and the gene/miRNA expression profiles were examined with Illumina Bead Chips and verified by real-time RT-PCR. Gene Ontology (GO) categories were determined, and pathway analysis was carried out using KEGG. miRNA target genes were predicted by implementing three computational analysis programs: TargetScanS, DIANA-microT and PicTar. Two hundred and forty-eight miRNAs and 3,631 genes were found to be significantly deregulated (gene, P<0.05; miRNA, P<0.01) in PTC tissues when compared with their matching normal thyroid tissues. hsa-miR-206 (target gene, MET), hsa-miR-299-3p (target gene, ITGAV), hsa-miR-101 (target gene, ITGA3), hsa-miR-103 (target gene, ITGA2), hsa-miR-222 (target genes, KIT and AXIN2), hsa-miR-15a (target genes, AXIN2 and FOXO1) and hsa-miR-221 (target gene, KIT) were identified. Together with the functions of the target genes, we further elucidated the role of miRNAs in papillary thyroid carcinogenesis and suggest the use of miRNAs as biomarkers for early diagnosis. Our findings provide the basis for future studies in the field of miRNA-based cancer therapy.
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