期刊
ONCOLOGY REPORTS
卷 30, 期 6, 页码 2593-2602出版社
SPANDIDOS PUBL LTD
DOI: 10.3892/or.2013.2778
关键词
alpha-tomatine; MCF-7; cell death; cholesterol
类别
资金
- Charles University in Prague [PRVOUK P37/01, SVV-2013-266901]
- European Social Fund
- state budget of the Czech Republic [CZ.1.07/2.3.00/30.0022]
- [GACR P303/12/P536]
In recent years, -tomatine has been studied for its anticancer activity. In the present study, we focused on the cytotoxic effect of -tomatine in the MCF-7 human breast adenocarcinoma cell line, its mechanism of action, biotransformation and stability in the culture medium. We observed an inhibition of cell proliferation and viability at concentrations of 6 and 9 M but then a recovery of cells occurred. The recovery was not caused by the biotransformation of -tomatine in MCF-7 cells, but by a substantial decrease in the concentration of -tomatine in the culture medium due to its binding with cholesterol. Regarding the mechanism of action of -tomatine, we observed no DNA damage, no changes in the levels of the proteins p53 and p21(WAF1/Cip1), and no apoptosis (neither activated caspase-8 and -9, nor sub-G1 peak, or morphological signs). We found a loss of ATP in -tomatine-treated cells. These results support the conclusion that -tomatine does not induce apoptosis in the MCF-7 cell line.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据