期刊
ONCOLOGY REPORTS
卷 30, 期 1, 页码 201-206出版社
SPANDIDOS PUBL LTD
DOI: 10.3892/or.2013.2456
关键词
glioma; OCT4; hypomethylation
类别
资金
- Youth Fund of the National Natural Science Foundation of China [81201975]
- Youth Fund of the Natural Science Foundation of Jiangsu Province [BK2012224]
- Natural Science Foundation of China Ministry of Health [2010-2-025]
- Natural Science Foundation of Jiangsu Department of Health [H201124]
- Six Major Human Resources Project of Jiangsu Province [2011-WS-065, 2010-WS-038]
- Natural Science Foundation of Jiangsu Colleges and Universities Grant [11KJB320010]
Glioma is the leading cause of tumor-related mortality in the central nervous system. There is increasing evidence that the self-renewal capacity of cancer cells is critical for the initiation, growth and recurrence of tumors. OCT4 is a transcription factor that plays a key role in regulating the self-renewal ability of embryonic stem cells. DNA methylation is involved in the regulation of OCT4 expression during the development and differentiation of embryonic stem cells and neural stem cells. In the present study, we reported that OCT4 was highly expressed in primary gliomas and its expression levels increased in parallel with pathological grades. BSP analysis showed that the methylation levels of OCT4 gene promoter and exon were significantly reduced in comparison with the normal group and were negatively correlated with OCT4 gene expression in primary gliomas. In vitro, OCT4 gene expression was upregulated following treatment by a demethylation reagent in glioma cell lines. Our findings suggest that OCT4 is epigenetically regulated by DNA hypomethylation in primary gliomas, which may provide evidence for the role of DNA methylation in tumor and may present a new direction for developing more powerful strategies to treat glioma in the clinic.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据