期刊
ONCOLOGY REPORTS
卷 24, 期 6, 页码 1599-1604出版社
SPANDIDOS PUBL LTD
DOI: 10.3892/or_00001023
关键词
HPV-16 E6; DNMT1; p53; cervical cancer
类别
资金
- Hong Kong Research Grants Council [466908, 467609]
The HPV-16 early proteins E6 and E7 are considered to function as oncoproteins in cervical cancer. DNA methyltransferase 1 (DNMT1) is one of the enzymes involved in epigenetic silencing of tumor suppressor genes. In the present study, the functional role and regulation of DNMT1 in HPV-16 E6 associated cervical cancer development were examined. Knockdown of E6 in HPV-16 positive human cervical carcinoma Si Ha and CaSki cells led to the increase in p53, repression of DNMT1 protein and promoter activity. Moreover, p53 knockdown increased the DNMT1 protein as well as promoter activity, indicating that p53 may mediate E6 upregulation of DNMT1. In addition, E6 knockdown induced growth retardation in SiHa cells, and the effect was partially reverted by DNMT1 overexpression. The results suggest that HPV-16 E6 may act through p53/DNMT1 to regulate the development of cervical cancer.
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