期刊
ONCOLOGY REPORTS
卷 22, 期 3, 页码 569-574出版社
SPANDIDOS PUBL LTD
DOI: 10.3892/or_00000473
关键词
brain tumors; gliomas; DNA synthesis; cell proliferation; genistein; RG2 model
类别
资金
- Karolinska Institute
- Minerva Foundation
- Swedish Medical Association
- Swedish Research Council
The effect of genistein, a protein tyrosine kinase and topoisomerase II inhibitor, on the DNA synthesis rate was studied in 21 human glioma specimens obtained at routine craniotomies for tumor resection. Ongoing DNA synthesis rate was determined by using a method based on the generation of tissue mini-units immediately after tumor resection and short incubation time (0-120 min) with [methyl-H-3]-thymidine. A 9-77% inhibition of DNA synthesis rate by 100 mu M genistein was observed in 18/21 of the glioma specimens. In these cases, the average percentage of inhibition was 55 +/- 20% (mean +/- SD, P<0.0001, Student's t-test) and the inhibitory effect was >50% in 12/18 of the cases. In 3 cases genistein increased the DNA synthesis rate. The inhibitory effect of genistein had a short-time onset and was concentration-dependent. Additional experiments in 4 cases showed that herbimycin A had no effect on DNA synthesis rate while etoposide inhibited similarly to that of genistein. Our results suggest that the effect of genistein on DNA synthesis rate in gliomas is independent of protein kinase inhibition and probably mediated by topoisomerase II inhibition. In the RG2 model, 50 mu M genistein inhibited ongoing DNA synthesis in glioma cells with little or no effect in normal tissue. The data also encourage further investigations on the therapeutic potential of genistein for gliomas.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据