期刊
ONCOLOGY
卷 75, 期 1-2, 页码 27-31出版社
KARGER
DOI: 10.1159/000151616
关键词
bombesin-like peptides; gastrin-releasing peptide receptor; cAMP signaling; glioblastoma; brain tumor
类别
资金
- National Council for Scientific and Technological Development (CNPq, Brazil) [400839/2005-9, 301578/2006-0]
- South American Office for Anticancer Drug Development (Porto Alegre, Brazil)
- Children's Cancer Institute (Porto Alegre, Brazil)
Increasing evidence indicates that gastrin-releasing peptide (GRP) acts as an autocrine growth factor for brain tumors. However, it remains unclear whether the cAMP/protein kinase A (PKA) signaling pathway plays a role in mediating the mitogenic effects of GRP. We show here that GRP combined with agents that stimulate the cAMP/PKA pathway promotes proliferation of human gliobastoma cells. Treatment with GRP combined with the adenylyl cyclase activator forskolin, the cAMP analog 8-Br-cAMP or the phosphodiesterase type IV inhibitor rolipram increased proliferation of U138-MG cells in vitro measured by MTT assay. None of the compounds had an effect when given alone. GRP receptor (GRPR) mRNA and protein expression in U138-MG cells was detected by reverse transcriptase polymerase chain reaction (RT-PCR) and immunohistochemistry. The results suggest that GRP and the GRPR interact with the cAMP/PKA signaling pathway in stimulating cancer cell proliferation. Copyright (C) 2008 S. Karger AG, Basel.
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