期刊
ONCOGENE
卷 31, 期 31, 页码 3569-3583出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/onc.2011.547
关键词
adhesion; breast cancer; EGF; HER2; hypoxia
资金
- National Cancer Institute [CA72981]
- M.D. Moross Institute for Cancer Research
- Kekst Family Institute for Medical Genetics
- Women's Health Research Center
- Bennett-Pritzker Endowment Fund
- Marvelle Koffler Program for Breast Cancer Research
- Harry and Jeanette Weinberg Women's Health Research Endowment
- Oprah Winfrey Biomedical Research Fund
- Arresto Biosciences
- European Commission
- German Research Foundation
The HER2/neu oncogene encodes a receptor-like tyrosine kinase whose overexpression in breast cancer predicts poor prognosis and resistance to conventional therapies. However, the mechanisms underlying aggressiveness of HER2 (human epidermal growth factor receptor 2)-overexpressing tumors remain incompletely understood. Because it assists epidermal growth factor (EGF) and neuregulin receptors, we overexpressed HER2 in MCF10A mammary cells and applied growth factors. HER2-overexpressing cells grown in extracellular matrix formed filled spheroids, which protruded outgrowths upon growth factor stimulation. Our transcriptome analyses imply a two-hit model for invasive growth: HER2-induced proliferation and evasion from anoikis generate filled structures, which are morphologically and transcriptionally analogous to preinvasive patients' lesions. In the second hit, EGF escalates signaling and transcriptional responses leading to invasive growth. Consistent with clinical relevance, a gene expression signature based on the HER2/EGF-activated transcriptional program can predict poorer prognosis of a subgroup of HER2-overexpressing patients. In conclusion, the integration of a three-dimensional cellular model and clinical data attributes progression of HER2-overexpressing lesions to EGF-like growth factors acting in the context of the tumor's microenvironment. Oncogene (2012) 31, 3569-3583; doi:10.1038/onc.2011.547; published online 5 December 2011
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