期刊
ONCOGENE
卷 31, 期 35, 页码 3989-3998出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/onc.2011.550
关键词
p27(Kip1); cyclin F; micronuclei; centrosome amplification
资金
- Team Science Grant [09KT-03-23121]
- Cortner Couch Chair
- Amandalee Weiss Foundation
- V Foundation
- Bankhead-Coley Post-doctoral Grant [IBD08-34209]
Mitotic catastrophe occurs when cells enter mitosis with damaged DNA or excess centrosomes. Cells overexpressing the centrosome protein CP110 or depleted of cyclin F, which targets CP110 for destruction, have more than two centrosomes and undergo mitotic catastrophe. Our studies show centrosome reduplication and mitotic catastrophe in osteosarcoma cells inducibly expressing a p27(Kip1) mutant (termed p27K) that binds cyclins but not cyclin-dependent kinases (CDKs). p27K inhibited cell proliferation but not CDK activity or cell cycle progression. It did not induce apoptosis; however, cells expressing p27K had more than two centrosomes and, indicative of mitotic catastrophe, irregularly shaped nuclei or multiple micronuclei. p27K interacted with cyclin F in vivo (as did endogenous p27(Kip1)) and displaced cyclin F from CP110. Depletion of CP110 rescued p27K-expressing cells from centrosome reduplication and mitotic catastrophe. Collectively, our data show that p27(Kip1) can perturb mitosis and suggest that it does so by sequestering cyclin F, which prevents its interaction with and the subsequent degradation of CP110, ultimately resulting in centrosome reduplication, mitotic catastrophe and abrogation of cell proliferation. Oncogene (2012) 31, 3989 3998; doi: 10.1038/onc.2011.550; published online 12 December 2011
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