期刊
ONCOGENE
卷 30, 期 12, 页码 1436-1448出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/onc.2010.509
关键词
vimentin; EMT; Axl
资金
- Academy of Finland
- Sigrid Juselius Foundation
- Emil Aaltonen Foundation
- Finnish Cancer Organisations
- EMBO
- University of Turku
- Turku University Foundation
- Finnish Concordia Fund
- Finnish Cultural Foundation
- Ida Montini Foundation
- Orion and Farmos Research Foundation
- K Albin Johansson's Foundation
- Scientific Foundation of Instrumentarium
- Turku Graduate School for Biomedical Sciences
Epithelial-to-mesenchymal transition (EMT) is a critical event in the progression toward cancer metastasis. The intermediate filament protein vimentin is an important marker of EMT and a requisite regulator of mesenchymal cell migration. However, it is not known how vimentin functionally contributes to cancer cell invasion. Here, we report that ectopic expression of oncogenic H-Ras-V12G and Slug induces vimentin expression and migration in pre-malignant breast epithelial cells. Conversely, vimentin expression is necessary for Slug-or H-Ras-V12G-induced EMT-associated migration. Furthermore, silencing of vimentin in breast epithelial cells results in specific changes in invasiveness-related gene expression including upregulation of RAB25 (small GTPase Rab25) and down-regulation of AXL (receptor tyrosine kinase Axl), PLAU (plasminogen activator, urokinase) and ITGB4 (integrin beta 4-subunit). Importantly, gene expression profiling analyses reveal that vimentin expression correlates positively/negatively with these genes also in multiple breast cancer cell lines and breast cancer patient samples. Focusing on the tyrosine kinase Axl, we show that induction of vimentin by EMT is associated with upregulation of Axl expression and that Axl enhances the migratory activity of pre-malignant breast epithelial cells. Using null and knock-down cells and overexpression models, we also show that regulation of breast cancer cell migration in two-and three-dimensional matrices by vimentin is Axl-dependent and that Axl functionally contributes to lung extravasation of breast cancer cells in mice. In conclusion, our data show that vimentin functionally contributes to EMT and is required for induction of Axl expression. Moreover, these results provide a molecular explanation for vimentin-dependent cancer cell migration during EMT by identifying Axl as a key proximal component in this process. Oncogene (2011) 30, 1436-1448; doi:10.1038/onc.2010.509; published online 8 November 2010
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