期刊
ONCOGENE
卷 29, 期 25, 页码 3691-3702出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/onc.2010.120
关键词
Myc; LSD1; transcription; histone; methylation; oxidative burst
资金
- Italian Association for Cancer Research (AIRC)
Myc is a transcription factor that significantly contributes to cancer progression by modulating the expression of important genes through binding to a DNA sequence, CACGTG, called E-box. We find that on Myc binding to chromatin, the lysine-demethylating enzyme, LSD1, triggers a transient demethylation of lysine 4 in the histone H3. In addition, we demonstrate that Myc binds and recruits LSD1 to the E-box chromatin and the formation of this complex is stimulated by cAMP-PKA. Demethylation by LSD1 produces H2O2, which locally oxidizes guanine and induces the recruitment of 8-oxoguanine-DNA glycosylase (OGG1) and of the nuclease Ape1 on the E-box chromatin. Inhibition of oxidation or silencing of LSD1, OGG1 or Ape1 significantly reduce transcription and inhibit mRNA accumulation of Myc-target genes. Collectively, these data highlight the role of transient LSD1-mediated demethylation of H3K4 leading to local DNA oxidation as driving force in the assembly of the Myc-induced transcription initiation complex. Oncogene (2010) 29, 3691-3702; doi: 10.1038/onc.2010.120; published online 26 April 2010
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