期刊
ONCOGENE
卷 28, 期 13, 页码 1626-1638出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/onc.2009.6
关键词
NF-kappa B; MMP9; H3K4 HMT; p100
资金
- University of Liege (ULg)
- FNRS
- TELEVIE
- Belgian Federation against Cancer
- Belgian State, Science Policy Office [IAP6/18]
Constitutive nuclear factor (NF)-kappa Ba ctivation in haematological malignancies is caused in several cases by loss of function mutations within the coding sequence of NF-kappa B inhibitory molecules such as I kappa B alpha or p100. Hut-78, a truncated form of p100, constitutively generates p52 and contributes to the development of T-cell lymphomas but the molecular mechanism underlying this oncogenic potential remains unclear. We show here that MMP9 gene expression is induced through the alternative NF-kappa B-activating pathway in fibroblasts and also on Hut-78 or p52 overexpression in fibroblasts as well as in lymphoma cells. p52 is critical for Hut-78-mediated MMP9 gene induction as a Hut-78 mutant as well as other truncated NF-kappa B2 proteins that are not processed into p52 failed to induce the expression of this metalloproteinase. Conversely, MMP9 gene expression is impaired in p52-depleted HUT-78 cells. Interestingly, MLL1 and MLL2 H3K4 methyltransferase complexes are tethered by p52 on the MMP9 but not on the I kappa B alpha promoter, and the H3K4 trimethyltransferase activity recruited on the MMP9 promoter is impaired in p52-depleted HUT-78 cells. Moreover, MLL1 and MLL2 are associated with Hut-78 in a native chromatin-enriched extract. Thus, we identified a molecular mechanism by which the recruitment of a H3K4 histone methyltransferase complex on the promoter of a NF-kappa B-dependent gene induces its expression and potentially the invasive potential of lymphoma cells harbouring constitutive activity of the alternative NF-kappa B-activating pathway.
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