期刊
TALANTA
卷 140, 期 -, 页码 88-95出版社
ELSEVIER
DOI: 10.1016/j.talanta.2015.03.025
关键词
Aptasensor; Surface Plasmon Resonance; Plastic optical fiber; Protein biomarkers; Vascular endothelial growth factor; Cancer
资金
- Province of Trento Grandi Progetti [2790 22/12/2006]
- POR Campania FSE
The introduction of new compact systems for sensitive, fast and simplified analysis is currently playing a substantial role in the development of point-of-care solutions aimed to assist both prognosis and diagnosis. Here we report a simple and low cost biosensor based on Surface Plasmon Resonance (SPR) taking advantage of a plastic optical fiber (POF) for the detection of Vascular endothelial growth factor (VEGF), selected as a circulating protein potentially associated with cancer. Our system is based onto two crucial aspects. By one hand, the functional layer which allows the transduction signal is based on DNA aptamers, short oligonucleotide sequences that bind to non-nucleic acid targets with high affinity and specificity. By the other hand, the light guiding structure is based on a POF with a planar gold layer as the sensing region, which is particularly suitable for bioreceptors implementation. The sensor revealed to be really useful in the interface characterization. The developed system is relatively easy to realize and could well address the development of a rapid, portable and low cost diagnostic platform, with a sensitivity in the nanomolar range. (C) 2015 Elsevier B.V. All rights reserved.
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