期刊
TALANTA
卷 141, 期 -, 页码 288-292出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2015.04.004
关键词
Synthesis; Thiol aromatic aldehyde; Antibody immobilization; Electrochemical immunosensor; Human IgG
资金
- National Natural Science Foundation of China [21342012, 21375037]
- Hunan Provincial Natural Science Foundation of China [11JJ3023, 15JJ3094]
- Science and Technology Department [13JJ2020]
- State Key Laboratory of Chemo/Biosensing and Chemometrics Foundation [KLCBTC MR 2011-05]
- Ministry of Education [KLCBTCMR2011-08]
- Youth Science Fund of Hunan Agricultural University [13QN04]
In this work, a novel sulfur-containing compound with aldehyde groups was synthesized, which was used to immobilize antibodies through the covalent bonding of the aldehyde groups with amino groups, in which no additional chemical cross-linker is required. Human IgG was used as a model analyte to fabricate the electrochemical immunosensor. Using the proposed immunosensor, IgG was detected within the range from 0.01 to 25 ng mL(-1) with a detection limit of 0.003 ng mL(-1) obtained by 3 S/N. The simple electrochemical immunosensor had a good specificity, stability and reproducibility. (C) 2015 Elsevier B.V. All rights reserved.
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