期刊
TALANTA
卷 131, 期 -, 页码 259-265出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2014.07.088
关键词
Magnetic; Thermoresponsive; Targeted temperature sensing
资金
- Natural Science Foundation of China (NSFC) [61275182, 61177033, 21104009]
- Science Foundation for The Excellent Youth Scholars of Southeast University
- Scientific Research Foundation of Graduate School of Southeast University [YBJJ1125]
- Scientific Innovation Research Foundation of College Graduate in Jiangsu Province [CXZZ12_0094]
- Fundamental Research Funds for the Central Universities
A magnetic fluorescent nano-thermometer is presented. To fabricate the nano-thermometer, magnetic nanoparticles (Fe3O4) were first encapsulated with a silica layer. Then a poly (N-isopropylacrylamide) (pNIPAM) copolymer shell with Rhodamine B isothiocyanate (RhBITC) embedded inside was further coated, which was denoted as the pNIPAM-co-RhBITC shell. Finally, gold nanoparticles were introduced onto the copolymer shell by in-situ growth method and the nano-thermometer (denoted as Fe3O4@SiO2@(pNIPAM-co-RhBITC)/Au) was obtained. The nano-thermometer shows dual responses to both magnetism and temperature. Specifically, the fluorescence intensity of the nano-thermometer decreases as the temperature increases, which makes the nano-thermometer suitable for intracellular temperature sensing. Using this nano-thermometer, temperature changes in live HeLa cells can be successfully detected. Moreover, due to the Fe3O4 component, magnetic field guided targeting can be realized, thus targeted temperature sensing can be achieved for living cells. Cellular temperature changes can be easily detected using the proposed nano-thermometer in the range of 26 degrees C to 41 degrees C with a sensitivity of -4.84%degrees C-1. (C) 2014 Elsevier B.V. All rights reserved.
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